Positive effects of Moringa oleifera and Moringa stenopetala seed and leaf extracts against selected bacteria


  • Grace J. Miller Taylor University
  • Kaley Necessary Indiana Wesleyan University
  • Robert Burchell Indiana Wesleyan University
  • Yui Iwase Indiana Wesleyan University
  • Nicole L. Lautensack Indiana Wesleyan University
  • Blake Russell Indiana Wesleyan University
  • Erik G. Holder Indiana Wesleyan University
  • Emma G. Knee Indiana Wesleyan University
  • W. Matthew Sattley Indiana Wesleyan University




Moringa oleifera, Moringa stenopetala, antibacterial, plant extract, disk diffusion test, minimum inhibitory concentration, medicinal plant


Moringa oleifera is hailed as the “miracle tree” for its impressive catalog of nutritional, medicinal, and water purification benefits. A (sub)tropical plant with a rapid growth rate (3–5 m in a single season), Moringa has proven beneficial in multiple ways in developing regions around the world. In addition to its high nutrient content and water clarifying properties, Moringa seed and leaf extracts have shown potential as natural antibacterial agents. Based on this, we anticipated that extracts from multiple species of Moringa would exhibit potentially useful antibacterial properties against a range of bacterial species. To explore this, both disk diffusion and minimum inhibitory concentration (MIC) culture techniques were employed to assess the inhibitory effects of seed and leaf extracts from M. oleifera and M. stenopetala against species of bacteria commonly used in research and teaching laboratories. Aqueous seed extracts from both Moringa species showed broad-spectrum activity but were especially effective at inhibiting the growth of Gram-positive bacteria, including species of Staphylococcus, Streptococcus, and Bacillus. Moringa leaf extracts also exhibited antibacterial activity, with ethanolic leaf extracts showing greater efficacy than aqueous leaf extracts in disk-diffusion assays. Temporary acidification (1 h at pH 2) of Moringa seed and leaf extracts had a detrimental effect on their antibacterial activity. MIC assays using Moringa leaf extracts also showed more pronounced inhibition of Gram-positive bacteria (MIC = 12.5% v/v) versus Gram-negative species (MIC = 25% v/v). These results are of particular relevance in tropical areas where pharmaceutical drugs are scarce but Moringa is widely available and often used as a nutritional supplement. Moreover, the rising threat of multi-drug resistant pathogens lends greater importance to the study of antibacterial plant products that ultimately may find application in the clinical setting.


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Adane, L., Teshome, M., & Tariku, Y. (2019) Isolation of compounds from root bark extracts of Moringa stenopetala and evaluation of their antibacterial activities. Journal of Pharmacognosy and Phytochemistry, 8, 4228–4244.

Aissani, N., Coroneo, V., Fattouch, S., & Caboni, P. (2012) Inhibitory effect of carob (Ceratonia siliqua) leaves methanolic extract on Listeria monocytogenes. Journal of Agricultural and Food Chemistry, 60, 9954–9958. https://pubs.acs.org/doi/pdf/10.1021/jf3029623

Alviano, D. S., Barreto, A. L. S., Dias, F. d. A., Rodrigues, I. d. A., dos Santos Rosa, M. d. S, Alviano, C. S., & de Araújo Soares, R. M. (2012) Conventional therapy and promising plant-derived compounds against trypanosomatid parasites. Frontiers in Microbiology, 3, 283. https://doi.org/10.3389/fmicb.2012.00283

Ambrose, O. M., Nguyen, T. T. M., & Nowicki, E. M. (2022) Antibacterial effects of bitter melon extract in combination with commonly prescribed antibiotics. Fine Focus, 8, 74–85

Arun, T., & Rao, P. (2011) Phytochemical screening and antibacterial activity of Moringa oleifera Lam. against Proteus mirabilis from urinary tract infected patients. International Journal of PharmTech Research, 3, 2118–2123.

Bauer, A. W., Kirby, W. M. M., Sherris, J. C., & Turck, M. (1966) Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, 45, 493–496.

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., & Yadav, A. (2013) Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. International Journal of Microbiology, 746165. https://doi.org/10.1155/2013/746165

Chekesa, B., & Mekonnen, Y. (2015) Antibacterial activity of Moringa stenopetala against some human pathogenic bacterial strains. Science, Technology and Arts Research Journal, 4, 190–198. https://doi.org/10.4314/star.v4i2.23

Eilert, U., Wolters, B., & Nahrstedt, A. (1981) The antibiotic principle of seeds of Moringa oleifera and Moringa stenopetala. Planta Medica Journal of Medicinal Plant Research, 42, 55–61.

European Committee on Antimicrobial Susceptibility Testing. (2023) Breakpoint Tables for Interpretation of MICs and Zone Diameters. Version 13.0. http://www.eucast.org

Ezugwu, R. I., & Chukwubike, C. (2014) Evaluation of the antimicrobial activity of Moringa oleifera leaves extract on Helicobacter pylori. IOSR Journal of Pharmacy and Biological Sciences, 9, 57–60.

Fahey, J. W. (2005) Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal, 1, 5.

Fontana, R., Caproni, A., Buzzi, R., Sicurella, M., Buratto, M., Salvatori, F., Pappadà, M., Manfredini, S., Baldisserotto, A., & Marconi, P. (2021) Effects of Moringa oleifera leaf extracts on Xanthomonas campestris pv. campestris. Microorganisms, 9, 2244. https://doi.org/10.3390/microorganisms9112244

Fontana R, Machi G, Caproni A, Sicurella, M., Buratto, M., Salvatori, F., Pappadà, M., Manfredini, S., Baldisserotto, A., & Marconi, P. (2022) Control of Erwinia amylovora growth by Moringa oleifera leaf extracts: In vitro and in planta effects. Plants, 11, 957. https://doi.org/10.3390/plants11070957

Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016) Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5, 49–56. https://doi.org/10.1016/j.fshw.2016.04.001

Hagos, Z., Teka, M. Z., Brhane, M. Y., Chaithanya, K. K., Gopalakrishnan, V. K., & Weletnsae, T. (2018) In vitro antibacterial activities of the methanolic and aqueous extracts of Moringa stenopetala leaves. Journal of Pharmacy Research, 12, 788–793.

Hayek, S. A., & Ibrahim, S. A. (2012) Antimicrobial activity of xoconostle pears (Opuntia matudae) against Escherichi coli O157:H7 in laboratory medium. International Journal of Microbiology, 368472. https://doi.org/10.1155/2012/368472

Jerri, H. A., Adolfsen, K. J., McCullough, L. R., Velegol, D., & Velegol, S. B. (2012) Antimicrobial sand via adsorption of cationic Moringa oleifera protein. Langmuir, 28, 2262–2268. https://doi.org/10.1021/la2038262

Kim, Y-J., & Kim, H. S. (2019) Screening Moringa species focused on development of locally available sustainable nutritional supplements. Nutrition Research and Practice, 13, 529–534. https://doi.org/10.4162/nrp.2019.13.6.529

Manilal, A., Sabu, K. R., & Shewangizaw, M. (2020) In vitro antibacterial activity of medicinal plants against biofilm-forming methicillin-resistant Staphylococcus aureus: efficacy of Moringa stenopetala and Rosmarinus officinalis extracts. Heliyon, 6, e03303. https://doi.org/10.1016/j.heliyon.2020.e03303

Miller, G. J., Cunningham, A. M. G., Iwase, Y., Lautensack, N. L., & Sattley, W. M. (2017) A laboratory activity demonstrating the antibacterial effects of extracts from two plant species, Moringa oleifera and Allium sativum (garlic). Journal of Microbiology and Biology Education, 18, 3.


Mubarek, A. K., Arbab, M. H., Mohammed, S. E., Kabbashi, A. S., Baraka, A. A., & Ahmed, N. M. (2022) Antimicrobial activity of Lepidium sativum against multi-drug resistant and sensitive Pseudomonas aeruginosa: A microbiological study from Khartoum State, Sudan. Al-Kindy College Medical Journal, 18, 96–100. https://doi.org/10.47723/kcmj.v18i2.807

Parrotta, J. A. (1993) Moringa oleifera Lam. Resedá, horseradish tree, Moringaceae, horseradish-tree family. SO-ITF-SM, United States Department of Agriculture, pp. 1–6.

Peixoto, J. R. O., Silva, G. C., Costa, R. A., de Sousa Fontenelle, J. L., Vieira, G. H. F., Filho, A. A. F., & Vieira, R. H. S. F. (2011) In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts. Asian Pacific Journal of Tropical Medicine, 4, 201–204. https://doi.org/10.1016/S1995-7645(11)60069-2

Posmontier, B. (2011) The medicinal qualities of Moringa oleifera. Holistic Nursing Practice, 25, 80–87. https://doi.org/10.1097/HNP.0b013e31820dbb27

Rahman, M. M., Sheikh, M. I., Sharmin, S. A., Islam, M. S., Rahman, M. A., Rahman, M. M., & Alam, M.F. (2009) Antibacterial activity of leaf juice and extracts of Moringa oleifera Lam. against some human pathogenic bacteria. CMU Journal of Natural Science, 8, 219–227. https://www.thaiscience.info/journals/Article/CMUJ/10557966.pdf

Razis, A. F. A., Ibrahim, M. D., & Kntayya, S. B. (2014) Health benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention, 15, 8571–8576. https://doi.org/10.7314/APJCP.2014.15.20.8571

Reygaert, W., & Jusufi, I. (2013) Green tea as an effective antimicrobial for urinary tract infections caused by Escherichia coli. Frontiers in Microbiology, 4, 162. https://doi.org/10.3389/fmicb.2013.00162

Seleshe, S., & Kang, S. N. (2019) In vitro antimicrobial activity of different solvent extracts from Moringa stenopetala leaves. Preventive Nutrition and Food Science, 24, 70–74. https://doi.org/10.3746/pnf.2019.24.1.70

Shalayel, M. H. F., Asaad, A. M., Qureshi, M. A., & Elhussein, A. B. (2017) Anti-bacterial activity of peppermint (Mentha piperita) extracts against some emerging multi-drug resistant human bacterial pathogens. Journal of Herbal Medicine, 7, 27–30. https://doi.org/10.1016/j.hermed.2016.08.003

Vieira, G. H. F., Mourão, J. A., Ângelo, Â. M., Costa, R. A., & Vieira, R. H. S. F. (2010) Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against gram-positive and gram-negative bacteria. Revista do Instituto de Medicina Tropical de São Paulo, 52, 129–132. https://doi.org/10.1590/S0036-46652010000300003



How to Cite

Miller, G., Necessary, K., Burchell, R., Iwase, Y., Lautensack, N., Russell, B., … Sattley, M. (2024). Positive effects of Moringa oleifera and Moringa stenopetala seed and leaf extracts against selected bacteria. Fine Focus, 10(1), 58–73. https://doi.org/10.33043/FF.10.1.58-73