Analyzing selected FDA approved drugs for effects on template switch mutagenesis in E. coli
DOI:
https://doi.org/10.33043/rq7a84axKeywords:
quasi-palindrome, mutagenesis, DNA replication inhibitors, DNA repairAbstract
Quasipalindromes (QPs) are DNA sequences that are imperfectly mirrored, known to form secondary structures like hairpins and cruciforms. These sites have been linked with a specific type of mutation called template-switch mutation (TSM). Certain drugs like 5-azaC, AZT, and ciprofloxacin are known to induce TSM. This study aims to assess the impact of five FDA-approved drugs—three antitumor drugs (CPT-11, Doxorubicin hydrochloride, and Gemcitabine hydrochloride) and two anti-inflammatories (Ibuprofen and Dexamethasone)—on template-switch mutagenesis. The findings reveal no statistically significant effects on frequency of mutations after treatment with Gemcitabine hydrochloride, Ibuprofen, Dexamethasone, or Doxorubicin hydrochloride. However, CPT-11 treatment showed a notable decrease in TSM, suggesting a potential role in disrupting the template-switching process.
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Copyright (c) 2025 April Swain, Emily Colburn, Kamila De Andrade, Pamela Rosales, Sydney Addorisio, Taylor Stuart, Angelina Galvao, Leigha Hubisz, Margaret Ouellette, Rana Hamssa, Laura Laranjo, Cara Pina
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